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Image Search Results
Journal: Nucleic Acids Research
Article Title: High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
doi: 10.1093/nar/gkn725
Figure Lengend Snippet: Difference in differential miRNA expression (ΔΔCq) between two neuroblastoma cell lines (NBL-S and IMR-32) as measured using Megaplex RT alone (ΔCq NP ) and Megaplex RT with pre-amplification (ΔCq P ). ΔΔCq values are plotted in function of the average expression of each miRNA in the two cell lines as quantified by Megaplex RT alone (average Cq NP ). Bar plots indicate the mean ΔΔCq value for miRNAs with an average Cq NP value ranging between 15–20, 20–25, 25–30 and 30–35. ( A ) Results when a Cq detection cutoff of 35 is applied. ( B ) Results when Cq detection cutoff is lowered to 30.
Article Snippet:
Techniques: Expressing, Amplification